Electrochemically reduced graphene oxide on CoCr biomedical alloy: Characterization, macrophage biocompatibility and hemocompatibility in rats with graphene and graphene oxide.
Identifieur interne : 000045 ( Main/Exploration ); précédent : 000044; suivant : 000046Electrochemically reduced graphene oxide on CoCr biomedical alloy: Characterization, macrophage biocompatibility and hemocompatibility in rats with graphene and graphene oxide.
Auteurs : M L Escudero [Espagne] ; I. Llorente [Espagne] ; B T Pérez-Maceda [Espagne] ; S San José-Pinilla [Espagne] ; L. Sánchez-L Pez [Espagne] ; R M Lozano [Espagne] ; S. Aguado-Henche [Espagne] ; C Clemente De Arriba [Espagne] ; M A Alobera-Gracia [Espagne] ; M C García-Alonso [Espagne]Source :
- Materials science & engineering. C, Materials for biological applications [ 1873-0191 ] ; 2020.
Descripteurs français
- KwdFr :
- Alliages de chrome (composition chimique), Alliages de chrome (pharmacologie), Animaux (MeSH), Graphite (composition chimique), Graphite (pharmacologie), Macrophages (métabolisme), Matériaux revêtus, biocompatibles (composition chimique), Matériaux revêtus, biocompatibles (pharmacologie), Mâle (MeSH), Oxydoréduction (MeSH), Rat Wistar (MeSH), Rats (MeSH), Souris (MeSH), Techniques électrochimiques (MeSH), Test de matériaux (MeSH).
- MESH :
- composition chimique : Alliages de chrome, Graphite, Matériaux revêtus, biocompatibles.
- métabolisme : Macrophages.
- pharmacologie : Alliages de chrome, Graphite, Matériaux revêtus, biocompatibles.
- Animaux, Mâle, Oxydoréduction, Rat Wistar, Rats, Souris, Techniques électrochimiques, Test de matériaux.
English descriptors
- KwdEn :
- Animals (MeSH), Chromium Alloys (chemistry), Chromium Alloys (pharmacology), Coated Materials, Biocompatible (chemistry), Coated Materials, Biocompatible (pharmacology), Electrochemical Techniques (MeSH), Graphite (chemistry), Graphite (pharmacology), Macrophages (metabolism), Male (MeSH), Materials Testing (MeSH), Mice (MeSH), Oxidation-Reduction (MeSH), Rats (MeSH), Rats, Wistar (MeSH).
- MESH :
- chemical , chemistry : Chromium Alloys, Coated Materials, Biocompatible, Graphite.
- chemical , pharmacology : Chromium Alloys, Coated Materials, Biocompatible, Graphite.
- metabolism : Macrophages.
- Animals, Electrochemical Techniques, Male, Materials Testing, Mice, Oxidation-Reduction, Rats, Rats, Wistar.
Abstract
Electrochemically reduced graphene oxide (ErGO) films on a biomedical grade CoCr alloy have been generated and characterized in order to study their possible application for use on joint prostheses. The electrodeposition process was performed by cyclic voltammetry. The characterization of the ErGO films on CoCr alloys by XPS revealed sp2 bonding and the presence of CO and CO residual groups in the graphene network. Biocompatibility studies were performed with mouse macrophages J774A.1 cell cultures measured by the ratio between lactate dehydrogenase and mitochondrial activities. An enhancement in the biocompatibility of the CoCr with the ErGO films was obtained, a result that became more evident as exposure time increased. Macrophages on the CoCr with the ErGO were well-distributed and conserved the characteristic cell shape. In addition, vimentin expression was unaltered in comparison with the control, results that indicated an improvement in the CoCr biocompatibility with the ErGO on the material surface. The in vivo response of graphene and graphene oxide was assessed by intraperitoneal injection in wistar rats. Red blood cells are one of the primary interaction sites so hemocompatibility tests were carried out. Rats inoculated with graphene and graphene oxide showed red blood cells of smaller size with a high content in hemoglobin.
DOI: 10.1016/j.msec.2019.110522
PubMed: 32228976
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Sanchez L Pez, L" sort="Sanchez L Pez, L" uniqKey="Sanchez L Pez L" first="L" last="Sánchez-L Pez">L. Sánchez-L Pez</name><affiliation wicri:level="2"><nlm:affiliation>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Lozano, R M" sort="Lozano, R M" uniqKey="Lozano R" first="R M" last="Lozano">R M Lozano</name><affiliation wicri:level="2"><nlm:affiliation>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Aguado Henche, S" sort="Aguado Henche, S" uniqKey="Aguado Henche S" first="S" last="Aguado-Henche">S. Aguado-Henche</name><affiliation wicri:level="3"><nlm:affiliation>Department of Surgery, Anatomy and Social Sciences, University of Alcalá, 28805 Alcalá de Henares, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Surgery, Anatomy and Social Sciences, University of Alcalá, 28805 Alcalá de Henares, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="De Arriba, C Clemente" sort="De Arriba, C Clemente" uniqKey="De Arriba C" first="C Clemente" last="De Arriba">C Clemente De Arriba</name><affiliation wicri:level="3"><nlm:affiliation>Department of Surgery, Anatomy and Social Sciences, University of Alcalá, 28805 Alcalá de Henares, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Surgery, Anatomy and Social Sciences, University of Alcalá, 28805 Alcalá de Henares, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Alobera Gracia, M A" sort="Alobera Gracia, M A" uniqKey="Alobera Gracia M" first="M A" last="Alobera-Gracia">M A Alobera-Gracia</name><affiliation wicri:level="2"><nlm:affiliation>College of Dentists and Stomatologists of León, Department of Biomedical Sciences, University of Leon, 24007 Leon, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>College of Dentists and Stomatologists of León, Department of Biomedical Sciences, University of Leon, 24007 Leon</wicri:regionArea><placeName><region nuts="2" type="communauté">Castille-et-León</region></placeName></affiliation></author><author><name sortKey="Garcia Alonso, M C" sort="Garcia Alonso, M C" uniqKey="Garcia Alonso M" first="M C" last="García-Alonso">M C García-Alonso</name><affiliation wicri:level="2"><nlm:affiliation>Department of Surface Engineering, Corrosion and Durability, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Avda. Gregorio del Amo 8, 28040 Madrid, Spain. Electronic address: crisga@cenim.csic.es.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Surface Engineering, Corrosion and Durability, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Avda. 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Gregorio del Amo 8, 28040 Madrid, Spain. Electronic address: escudero@cenim.csic.es.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Surface Engineering, Corrosion and Durability, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Avda. Gregorio del Amo 8, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Llorente, I" sort="Llorente, I" uniqKey="Llorente I" first="I" last="Llorente">I. Llorente</name><affiliation wicri:level="2"><nlm:affiliation>Department of Surface Engineering, Corrosion and Durability, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Avda. Gregorio del Amo 8, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Surface Engineering, Corrosion and Durability, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Avda. Gregorio del Amo 8, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Perez Maceda, B T" sort="Perez Maceda, B T" uniqKey="Perez Maceda B" first="B T" last="Pérez-Maceda">B T Pérez-Maceda</name><affiliation wicri:level="2"><nlm:affiliation>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Jose Pinilla, S San" sort="Jose Pinilla, S San" uniqKey="Jose Pinilla S" first="S San" last="José-Pinilla">S San José-Pinilla</name><affiliation wicri:level="2"><nlm:affiliation>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Sanchez L Pez, L" sort="Sanchez L Pez, L" uniqKey="Sanchez L Pez L" first="L" last="Sánchez-L Pez">L. Sánchez-L Pez</name><affiliation wicri:level="2"><nlm:affiliation>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Lozano, R M" sort="Lozano, R M" uniqKey="Lozano R" first="R M" last="Lozano">R M Lozano</name><affiliation wicri:level="2"><nlm:affiliation>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Aguado Henche, S" sort="Aguado Henche, S" uniqKey="Aguado Henche S" first="S" last="Aguado-Henche">S. Aguado-Henche</name><affiliation wicri:level="3"><nlm:affiliation>Department of Surgery, Anatomy and Social Sciences, University of Alcalá, 28805 Alcalá de Henares, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Surgery, Anatomy and Social Sciences, University of Alcalá, 28805 Alcalá de Henares, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="De Arriba, C Clemente" sort="De Arriba, C Clemente" uniqKey="De Arriba C" first="C Clemente" last="De Arriba">C Clemente De Arriba</name><affiliation wicri:level="3"><nlm:affiliation>Department of Surgery, Anatomy and Social Sciences, University of Alcalá, 28805 Alcalá de Henares, Madrid, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Surgery, Anatomy and Social Sciences, University of Alcalá, 28805 Alcalá de Henares, Madrid</wicri:regionArea><placeName><settlement type="city">Madrid</settlement><region nuts="2" type="region">Communauté de Madrid</region></placeName></affiliation></author><author><name sortKey="Alobera Gracia, M A" sort="Alobera Gracia, M A" uniqKey="Alobera Gracia M" first="M A" last="Alobera-Gracia">M A Alobera-Gracia</name><affiliation wicri:level="2"><nlm:affiliation>College of Dentists and Stomatologists of León, Department of Biomedical Sciences, University of Leon, 24007 Leon, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>College of Dentists and Stomatologists of León, Department of Biomedical Sciences, University of Leon, 24007 Leon</wicri:regionArea><placeName><region nuts="2" type="communauté">Castille-et-León</region></placeName></affiliation></author><author><name sortKey="Garcia Alonso, M C" sort="Garcia Alonso, M C" uniqKey="Garcia Alonso M" first="M C" last="García-Alonso">M C García-Alonso</name><affiliation wicri:level="2"><nlm:affiliation>Department of Surface Engineering, Corrosion and Durability, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Avda. Gregorio del Amo 8, 28040 Madrid, Spain. Electronic address: crisga@cenim.csic.es.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Surface Engineering, Corrosion and Durability, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Avda. Gregorio del Amo 8, 28040 Madrid</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté de Madrid</region></placeName></affiliation></author></analytic><series><title level="j">Materials science & engineering. C, Materials for biological applications</title><idno type="eISSN">1873-0191</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Chromium Alloys (chemistry)</term><term>Chromium Alloys (pharmacology)</term><term>Coated Materials, Biocompatible (chemistry)</term><term>Coated Materials, Biocompatible (pharmacology)</term><term>Electrochemical Techniques (MeSH)</term><term>Graphite (chemistry)</term><term>Graphite (pharmacology)</term><term>Macrophages (metabolism)</term><term>Male (MeSH)</term><term>Materials Testing (MeSH)</term><term>Mice (MeSH)</term><term>Oxidation-Reduction (MeSH)</term><term>Rats (MeSH)</term><term>Rats, Wistar (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alliages de chrome (composition chimique)</term><term>Alliages de chrome (pharmacologie)</term><term>Animaux (MeSH)</term><term>Graphite (composition chimique)</term><term>Graphite (pharmacologie)</term><term>Macrophages (métabolisme)</term><term>Matériaux revêtus, biocompatibles (composition chimique)</term><term>Matériaux revêtus, biocompatibles (pharmacologie)</term><term>Mâle (MeSH)</term><term>Oxydoréduction (MeSH)</term><term>Rat Wistar (MeSH)</term><term>Rats (MeSH)</term><term>Souris (MeSH)</term><term>Techniques électrochimiques (MeSH)</term><term>Test de matériaux (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Chromium Alloys</term><term>Coated Materials, Biocompatible</term><term>Graphite</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Chromium Alloys</term><term>Coated Materials, Biocompatible</term><term>Graphite</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Alliages de chrome</term><term>Graphite</term><term>Matériaux revêtus, biocompatibles</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Macrophages</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Macrophages</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Alliages de chrome</term><term>Graphite</term><term>Matériaux revêtus, biocompatibles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Electrochemical Techniques</term><term>Male</term><term>Materials Testing</term><term>Mice</term><term>Oxidation-Reduction</term><term>Rats</term><term>Rats, Wistar</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Mâle</term><term>Oxydoréduction</term><term>Rat Wistar</term><term>Rats</term><term>Souris</term><term>Techniques électrochimiques</term><term>Test de matériaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Electrochemically reduced graphene oxide (ErGO) films on a biomedical grade CoCr alloy have been generated and characterized in order to study their possible application for use on joint prostheses. The electrodeposition process was performed by cyclic voltammetry. The characterization of the ErGO films on CoCr alloys by XPS revealed sp2 bonding and the presence of CO and CO residual groups in the graphene network. Biocompatibility studies were performed with mouse macrophages J774A.1 cell cultures measured by the ratio between lactate dehydrogenase and mitochondrial activities. An enhancement in the biocompatibility of the CoCr with the ErGO films was obtained, a result that became more evident as exposure time increased. Macrophages on the CoCr with the ErGO were well-distributed and conserved the characteristic cell shape. In addition, vimentin expression was unaltered in comparison with the control, results that indicated an improvement in the CoCr biocompatibility with the ErGO on the material surface. The in vivo response of graphene and graphene oxide was assessed by intraperitoneal injection in wistar rats. Red blood cells are one of the primary interaction sites so hemocompatibility tests were carried out. Rats inoculated with graphene and graphene oxide showed red blood cells of smaller size with a high content in hemoglobin.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32228976</PMID><DateCompleted><Year>2020</Year><Month>12</Month><Day>24</Day></DateCompleted><DateRevised><Year>2020</Year><Month>12</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-0191</ISSN><JournalIssue CitedMedium="Internet"><Volume>109</Volume><PubDate><Year>2020</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Materials science & engineering. C, Materials for biological applications</Title><ISOAbbreviation>Mater Sci Eng C Mater Biol Appl</ISOAbbreviation></Journal><ArticleTitle>Electrochemically reduced graphene oxide on CoCr biomedical alloy: Characterization, macrophage biocompatibility and hemocompatibility in rats with graphene and graphene oxide.</ArticleTitle><Pagination><MedlinePgn>110522</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0928-4931(18)32360-9</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.msec.2019.110522</ELocationID><Abstract><AbstractText>Electrochemically reduced graphene oxide (ErGO) films on a biomedical grade CoCr alloy have been generated and characterized in order to study their possible application for use on joint prostheses. The electrodeposition process was performed by cyclic voltammetry. The characterization of the ErGO films on CoCr alloys by XPS revealed sp2 bonding and the presence of CO and CO residual groups in the graphene network. Biocompatibility studies were performed with mouse macrophages J774A.1 cell cultures measured by the ratio between lactate dehydrogenase and mitochondrial activities. An enhancement in the biocompatibility of the CoCr with the ErGO films was obtained, a result that became more evident as exposure time increased. Macrophages on the CoCr with the ErGO were well-distributed and conserved the characteristic cell shape. In addition, vimentin expression was unaltered in comparison with the control, results that indicated an improvement in the CoCr biocompatibility with the ErGO on the material surface. The in vivo response of graphene and graphene oxide was assessed by intraperitoneal injection in wistar rats. Red blood cells are one of the primary interaction sites so hemocompatibility tests were carried out. Rats inoculated with graphene and graphene oxide showed red blood cells of smaller size with a high content in hemoglobin.</AbstractText><CopyrightInformation>Copyright © 2019 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Escudero</LastName><ForeName>M L</ForeName><Initials>ML</Initials><AffiliationInfo><Affiliation>Department of Surface Engineering, Corrosion and Durability, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Avda. Gregorio del Amo 8, 28040 Madrid, Spain. Electronic address: escudero@cenim.csic.es.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Llorente</LastName><ForeName>I</ForeName><Initials>I</Initials><AffiliationInfo><Affiliation>Department of Surface Engineering, Corrosion and Durability, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Avda. Gregorio del Amo 8, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pérez-Maceda</LastName><ForeName>B T</ForeName><Initials>BT</Initials><AffiliationInfo><Affiliation>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>José-Pinilla</LastName><ForeName>S San</ForeName><Initials>SS</Initials><AffiliationInfo><Affiliation>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sánchez-López</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lozano</LastName><ForeName>R M</ForeName><Initials>RM</Initials><AffiliationInfo><Affiliation>Cell-Biomaterial Recognition Lab. Department of Cellular and Molecular Biology, Centro de Investigaciones Biológicas (CIB-CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aguado-Henche</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Surgery, Anatomy and Social Sciences, University of Alcalá, 28805 Alcalá de Henares, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>de Arriba</LastName><ForeName>C Clemente</ForeName><Initials>CC</Initials><AffiliationInfo><Affiliation>Department of Surgery, Anatomy and Social Sciences, University of Alcalá, 28805 Alcalá de Henares, Madrid, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alobera-Gracia</LastName><ForeName>M A</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>College of Dentists and Stomatologists of León, Department of Biomedical Sciences, University of Leon, 24007 Leon, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>García-Alonso</LastName><ForeName>M C</ForeName><Initials>MC</Initials><AffiliationInfo><Affiliation>Department of Surface Engineering, Corrosion and Durability, Centro Nacional de Investigaciones Metalúrgicas (CENIM-CSIC), Avda. Gregorio del Amo 8, 28040 Madrid, Spain. Electronic address: crisga@cenim.csic.es.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>12</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Mater Sci Eng C Mater Biol Appl</MedlineTA><NlmUniqueID>101484109</NlmUniqueID><ISSNLinking>0928-4931</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002858">Chromium Alloys</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020099">Coated Materials, Biocompatible</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C000628730">graphene oxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>7782-42-5</RegistryNumber><NameOfSubstance UI="D006108">Graphite</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002858" MajorTopicYN="Y">Chromium Alloys</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020099" MajorTopicYN="Y">Coated Materials, Biocompatible</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055664" MajorTopicYN="Y">Electrochemical Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006108" MajorTopicYN="Y">Graphite</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008264" MajorTopicYN="N">Macrophages</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008422" MajorTopicYN="Y">Materials Testing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017208" MajorTopicYN="N">Rats, Wistar</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biocompatibility</Keyword><Keyword MajorTopicYN="N">Blood red cell</Keyword><Keyword MajorTopicYN="N">Graphene</Keyword><Keyword MajorTopicYN="N">Macrophages</Keyword><Keyword MajorTopicYN="N">Rats</Keyword><Keyword MajorTopicYN="N">Reduced graphene oxide</Keyword></KeywordList><CoiStatement>Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>08</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>12</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>12</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>12</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32228976</ArticleId><ArticleId IdType="pii">S0928-4931(18)32360-9</ArticleId><ArticleId IdType="doi">10.1016/j.msec.2019.110522</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country><region><li>Castille-et-León</li><li>Communauté de Madrid</li></region><settlement><li>Madrid</li></settlement></list><tree><country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Escudero, M L" sort="Escudero, M L" uniqKey="Escudero M" first="M L" last="Escudero">M L Escudero</name></region><name sortKey="Aguado Henche, S" sort="Aguado Henche, S" uniqKey="Aguado Henche S" first="S" last="Aguado-Henche">S. Aguado-Henche</name><name sortKey="Alobera Gracia, M A" sort="Alobera Gracia, M A" uniqKey="Alobera Gracia M" first="M A" last="Alobera-Gracia">M A Alobera-Gracia</name><name sortKey="De Arriba, C Clemente" sort="De Arriba, C Clemente" uniqKey="De Arriba C" first="C Clemente" last="De Arriba">C Clemente De Arriba</name><name sortKey="Garcia Alonso, M C" sort="Garcia Alonso, M C" uniqKey="Garcia Alonso M" first="M C" last="García-Alonso">M C García-Alonso</name><name sortKey="Jose Pinilla, S San" sort="Jose Pinilla, S San" uniqKey="Jose Pinilla S" first="S San" last="José-Pinilla">S San José-Pinilla</name><name sortKey="Llorente, I" sort="Llorente, I" uniqKey="Llorente I" first="I" last="Llorente">I. Llorente</name><name sortKey="Lozano, R M" sort="Lozano, R M" uniqKey="Lozano R" first="R M" last="Lozano">R M Lozano</name><name sortKey="Perez Maceda, B T" sort="Perez Maceda, B T" uniqKey="Perez Maceda B" first="B T" last="Pérez-Maceda">B T Pérez-Maceda</name><name sortKey="Sanchez L Pez, L" sort="Sanchez L Pez, L" uniqKey="Sanchez L Pez L" first="L" last="Sánchez-L Pez">L. Sánchez-L Pez</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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